An Immune Atlas of Clear Cell Renal Cell Carcinoma

نویسندگان

  • Stéphane Chevrier
  • Jacob Harrison Levine
  • Vito Riccardo Tomaso Zanotelli
  • Karina Silina
  • Daniel Schulz
  • Marina Bacac
  • Carola Hermine Ries
  • Laurie Ailles
  • Michael Alexander Spencer Jewett
  • Holger Moch
  • Maries van den Broek
  • Christian Beisel
  • Michael Beda Stadler
  • Craig Gedye
  • Bernhard Reis
  • Dana Pe’er
  • Bernd Bodenmiller
چکیده

Immune cells in the tumor microenvironment modulate cancer progression and are attractive therapeutic targets. Macrophages and T cells are key components of the microenvironment, yet their phenotypes and relationships in this ecosystem and to clinical outcomes are ill defined. We used mass cytometry with extensive antibody panels to perform in-depth immune profiling of samples from 73 clear cell renal cell carcinoma (ccRCC) patients and five healthy controls. In 3.5 million measured cells, we identified 17 tumor-associated macrophage phenotypes, 22 T cell phenotypes, and a distinct immune composition correlated with progression-free survival, thereby presenting an in-depth human atlas of the immune tumor microenvironment in this disease. This study revealed potential biomarkers and targets for immunotherapy development and validated tools that can be used for immune profiling of other tumor types.

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عنوان ژورنال:

دوره 169  شماره 

صفحات  -

تاریخ انتشار 2017